Abstract: OBJECTIVE To study the distribution of pathogenic bacteria and drug sensitivity of wound infection in open fracture patients, so as to reduce infection rate. METHODS A total of 160 wound infected patients with open fractures from Jan. 2012 to Jun. 2015 were selected. The pathogenic bacteria were isolated and identified, and the pathogenic bacteria distribution and drug resistance of wound infection in open fracture patients were explored. The data were analyzed by SPSS 17.0. RESULTS Totally 160 strains of pathogenic bacteria were isolated from 160 cases of infected patients, including 78 strains of gram-positive bacteria, accounting for 48.75%, 60 strains of gram-negative bacteria, accounting for 37.5%, and 22 strains of fungi, accounting for 13.75%. The drug resistant rates of gram-positive bacteria to ampicillin/sulbactam were high which were 100%, and low to nitrofurantoin, vancomycin and teicoplanin, which were 0. CONCLUSION The pathogenic bacteria of open fracture patients with wound infection were mainly gram-positive bacteria. It should strengthen aseptic operation, and rational use of antimicrobial drugs.