Abstract: OBJECTIVE To investigate the clinical effects tuberculosis infection T cells (TSPOT) detection in tuberculous meningitis, so as to provide further theoretical guidance and basis for the clinical diagnosis of tuberculous meningitis. METHODS A total of 150 patients with suspected tuberculous meningitis treated in hospital in Nov. 2013 to Oct. 2015 were selected, who were randomly divided into experimental and control groups based on the random number table with 75 cases of each group. Peripheral TSPOT was used in patients in experimental group, and TST was used in control group. The results in two groups were compared and statistically analyzed by SPSS 16.0. RESULTS A total of 58 cases were diagnosed as tuberculous meningitis in experimental group, and 50 cases with positive results tested by TSPOT, positive rate 86.2%. While 47 cases were diagnosed as tuberculous meningitis in control group, and 19 cases with positive results tested by TST, positive rate 40.4%. Difference between two test results was significantly (P<0.05). CONCLUSION This study shows that tuberculosis infection T cell test has a high sensitivity for tuberculous meningitis, which has a significantly better clinical detection effect than TST test, so it could be used for clinical diagnosis of tuberculous meningitis to further provide a reference.