Abstract: OBJECTIVE To analyze the correlation of Human papillomavirus (HPV) infection rate and the ILT4 level in patients with ovarian cancer detected by in situ hybridization method, so as to provide reference for clinical treatment of ovarian cancer. METHODS A total of 189 cases of normal ovarian and ovarian benign epithelial tumor resection specimens from Jul. 2012 to Dec. 2012 were studied, including 43 cases of normal ovarian tissue samples, 43 cases of ovarian benign epithelial tumor (ovarian cystadenoma) specimens, 52 cases of ovarian cancer specimens, and 51 cases of borderline tumor specimen. ILT4 levels of these four specimens were detected, and the correlation between ILT4 and HPV in ovarian cancer was analyzed using SPSS 18.0. RESULTS The HPV16/18 infection rate of ovarian cancer and that in borderline tumors had no significant difference, but significantly higher than that in ovarian cystadenoma and normal ovarian tissue(P<0.05). ILT4 positive expression and HPV16/18 infection had good positive correlation. CONCLUSION ILT4 has a positive correlation with ovarian cancer HPV16/18 infection and may promote HPV16/18 infection of ovarian cancer.