Abstract: OBJECTIVE To investigate the clinical diagnosis value of serum tumor necrosis factor(TNF-α), interleukin-6(IL-6) and C-reactive protein (CRP) in patients with bone infections, so as to provide references for clinical diagnosis of bone infections. METHODS A total of 123 cases of patients with suspected bone infections from Feb. 2013 to Oct. 2015 were selected, and divided into bone infection group (n=87) and bone metastasis group (n=36) according to the pathology, and another 40 cases of healthy physical examination personnel were selected as control group. The serum levels of TNF-α, IL-6 and CRP of the groups were detected and compared. RESULTS The serum levels of TNF-α, IL-6 and CRP of bone infection group were (215.64±72.17) ng/L, (879.56±143.15) ng/L and (87.64±34.25) mg/L, respectively, which were significantly higher than those of control group, and the serum levels of IL-6 and CRP of bone infection group were significantly higher than those of bone metastasis group (P<0.05). CONCLUSION There are varying degrees of changes ofTNF-α, IL-6 and CRP during the inflammation of bone infection, these indexes have important significance for predicting the occurrence and classification of bone infection, and the clinical reference value of CRP was the highest.