Abstract: OBJECTIVE To expolre the status postoperative infection in children with perforated appendicitis after laparoscopic operation, and to analyze the pathogenic bacteria in children with infection, so as to provide the basis for clinical infection prevention and control. METHODS A total of 146 cases of children with perforated appendicitis in our hospital from Oct. 2013 to Oct. 2015 were randomly divided into observation group with 72 cases and control group with 74 cases according to the random digital table method. The observation group were treated with laparoscopic surgery, and the control group were treated with conventional open operation. The rates of postoperative infection of the two groups were observed and compared, and the pathogenic bacteria in children with infection was analyzed. Data were analyzed by SPSS 19.0. RESULTS The incidence of complications and the incidence of infection in the observation group were 1.39% and 5.56%, control group 3.75% and 24.32% respectively, so there was no significant difference in the former comparison, but there was significant difference in the latter comparison (P<0.05). A total of 17 cases patients were got infection, and there were 26 strains of pathogens were detected, mainly gram-negative bacilli, 27 strains, accounting for 65.38%. CONCLUSION The use of laparoscopic surgery can effectively reduce the trauma of perforated appendicitis in children with perforated appendicitis, and promote rapid recovery and reduce the infection and complications, and in the perioperation period aseptic operation should be strictly followed, and especially gram negative bacteria should be increased control efforts.