Abstract: OBJECTIVE To analyze the pathogenic features of viral myocarditis, so as to provide the reference for clinical diagnosis and treatment. METHODS A total of 210 cases of viral myocarditis patients in our hospital from Feb. 2010 to Sep. 2014 were enrolled in this study as observation group and another 210 cases of healthy subjects at the same period in our hospital were taken as control group. The changes of myocardial enzymes levels were compared between these two groups. SPSS 17.0 was used for statistic analysis. RESULTS A total of 210 strains of pathogens isolated from the patients in observation group, mainly 119 strains of RNA virus (56.7%). The patients in observation group also showed abnormal characteristics in ECG, mainly from 26 cases (12.4%)of patients with lengthened interval in QT. The creatine kinase, creatine kinase isozyme, lactate dehydrogenase and α- hydroxybutyric acid in serum from observation group were significantly higher than that in control group (P<0.05). CONCLUSION RNA virus is the main pathogen in children with viral myocarditis with obvious abnormal ECG and myocardial enzymes.