Abstract: OBJECTIVE To study the effect of human beta-defensin 3 (rHBD3)restraining pathogenic bacteria on surface of maxillofacial silicone elastomer and alkaline phosphatase (ALP) activity, so as to provide reference for clinical research. METHODS A total of 100 patients with oral and maxillofacial defect were included from Jan. 2012 to Aug. 2015 and treated by maxillofacial silicone elastomer. They were divided into observation group and control group by random number table each with 50 cases. Patients in observation group were given rHBD3 disinfection, and patients in control group were given conventional disinfection. Chewing function was compared after 6 months. RESULTS The total effective rate of chewing function in observation group was 100.0%, which was significeaty higher than 92.0% in control group (P<0.05). The ALP activity after treatment in control group was (138.44±37.45)μg/L, which was higher than (95.39±32.54)μg/L before treatment and (95.45±39.76)μg/L after treatment in observation group. The difference was statistically significant (P<0.05). CONCLUSION RHBD3 could give a better inhibit pathogen infection for maxillofacial silicone elastomer, which also play an important role on postoperative function recovery, worth to promote in clinic.