Abstract: OBJECTIVE To observe the change situation of serum and urine oxidative stress indexes of patients with diabetes combined with urinary tract infection,in order to provide reference for the intervention measure. METHODS A total of 54 patients with diabetes combined with urinary tract infection in hospital from Jun. 2014 to Nov. 2015 were selected as the group A,another 54 patients with patients with diabetes and without infection as the group B,another 54 patients with urinary tract infection only as the group C,another 54 healthy persons as the group D,then the serum and urine oxidative stress indexes of four groups were compared. SAS 6.0 was used for the data analysis. RESULTS The serum and urine reactive oxygen and melatonin in group A were respectively (72.31±5.80)nU/ml,(45.68±5.20)ng/L and(63.74±4.98)nU/ml,(35.28±4.37a)ng/L,and they were all lower than the other three groups, group B and C lower than group D, and the differences were all significant(P＜0.05); The serum and urine MDA and MT levels of group A with different glycated hemoglobin levels were lower than those in group B, but MDA was higher than group B (P＜0.05). The serum and urine SOD and MT levels in group A different infection sites patients were lower than group C, but MDA was higher than group C(P＜0.05). CONCLUSION The change of serum and urine oxidative stress indexes of patients with diabetes combined with urinary tract infection are greater,and the influence of glycosylated hemoglobins and infection sites for the serum and urine indexes are both greater too.