Abstract: OBJECTIVE To investigate the pathogenic bacteria distribution and preventive measures of infective endocarditis in patients, so as to improve the clinical efficacy to infective endocarditis. METHODS A total of 348 cases of patients with infective endocarditis in hospital from Nov. 2014 to Oct. 2015 were selected to analyze the pathogenic bacteria distribution and drug resistance, using K-B disk diffusion method for drug sensitivity test, and based on the standard of National Committee for Clinical Laboratory Standards (NCCLS) to analyze the inspection results. RESULTS From the 348 specimens, a total of 276 were positive, and the positive rate was 79.31%. A total of 294 strains of pathogenic bacteria were isolated, mainly gram-positive bacteria, 209 strains, accounting for 71.09%. The drug resistant rates of Stenotrophomonas maltophilia and Escherichia coli to gentamicin were 52.38% and 52.78%. The drug resistant rate of Streptococcus to penicillin was very high, 94.44%, and Staphylococcus aureus to penicillin was 92.31%. CONCLUSION Gram positive bacteria are the main pathogens of infective endocarditis. Rational antimicrobial agents should be used in clinical treatment according to the results of the drug sensitivity test.