Abstract: OBJECTIVE To investigate the relationship between serum PCT, hs-CRP and endotoxin and the types of bacterial infection and conditions in patients with sepsis caused by bacterial bloodstream infection. METHODS Eighty patients with sepsis diagnosed by Henan Provincial People’s Hospital from Feb. to May 2018 were selected as the case group and 80 volunteers of the same period underwent physical examination were used as the control group. The serum PCT, hs-CRP and endotoxin levels of subjects in two groups were compared, and the serum PCT, hs-CRP and endotoxin levels of subjects in case group were analyzed according to the pathogen type. The relationship between serum PCT, hs-CRP, endotoxin and APACHE II score, SOFA score was analyzed by linear correlation analysis. RESULTS The serum PCT, hs-CRP and endotoxin in the case group were（1.22±0.40） μg/L,（86.14±24.71） mg/L and（11.39±4.39） ng/L, respectively, significantly higher than those in control group（P＜0.05）. Serum PCT, hs-CRP and endotoxin in sepsis patients with gram-positive bacterial infection were（0.75±0.32） μg/L,（65.40±22.33） mg/L and（6.77±3.95） ng/L, respectively, significantly higher than those in sepsis patients with gram-negative bacterial infection（P＜0.05）. The APACHE II score and SOFA score of sepsis patients infected with Gram-positive bacteria were（9.44±3.02） and（26.81±4.20）, respectively, lower than those infected with Gram-negative bacteria. The APACHE II score and SOFA score of patients with sepsis were positively correlated with serum PCT, hs-CRP and endotoxin levels（P＜0.001）. CONCLUSION Serum PCT, hs-CRP, and endotoxin levels in patients with sepsis caused by Gram-negative bacterial infection were more significant, and the relationship with the severity of the patient’s condition was more significant.