Abstract: OBJECTIVE To investigate the diagnostic value of interleukin-6（IL-6）, procalcitonin（PCT） and activin A（ACTA） in post-chemotherapy granulocytopenia infection of patients with acute leukemia stage. METHODS From Mar. 2016 to Jan. 2019, 192 patients with acute leukemia at the stage of granulocytopenia after chemotherapy admitted in the first affilicated hospital of Hainan medical college were selected as the study subjects, and were divided into 80 cases of the infection group and 112 casesof non-infection group according to whether they were co-infected after chemotherapy.The expression levels of serum IL-6, PCT and ACTA were compared between the two groups. ROC curve method and Logistic regression were used to analyze the diagnostic value of IL-6, PCT and activin-a in post-chemotherapy infection of patients with acute leukemia. RESULTS Totally 80 cases of nosocomial infection occurred in 192 patients with acute leukemia after chemotherapy, the infection rate was 41.67%. The main infection site was upper respiratory tract. A total of 84 samples were collected from 80 infected patients, and 96 strains of pathogenic bacteria were isolated, of which 58 strains of Gram-negative bacteria accounted for 60.42%, 32 strains of Gram-positive bacteria accounted for 33.33%, 6 strains of fungi accounted for 6.25%, dominated by Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Staphylococcus aureus. The serum concentrations of IL-6, PCT and activin-a in the infection group were（7.23±2.01） ng/L,（2.68±1.08） ng/mL and（0.56±0.17） ng/mL, respectively, significantly higher than those in the non-infection group（P＜0.001）. The ROC curve analysis showed that the AUC predicted by IL-6, PCT and ACTA was 0.859、0.801 and 0.916, respectively. CONCLUSION The early diagnosis of IL-6, PCT and ACTA for the granulocyte deficiency infection in patients with acute leukemia after chemotherapy had higher value and certain clinical value.