Abstract: OBJECTIVE To analyze the molecular prevalence characteristics of clinical isolated hypertrophic Klebsiella pneumoniae（HMKP） and provide laboratory data for clinical control of the spread of such bacteria. METHODS HMKP isolated from clinical patients in a teaching hospital for the first time from Apr. 2017 to May 2019 were collected. Six common high virulence serotype genes（K1, K2, K5, K20, K54, K57） and 9 virulence genes（rmpA, kfu, Aerobactin, iroN, entB, ybtS, fimH, mrkD, allS） were amplified by PCR. Multi-locus sequence typing（MLST） were used to analyze the homology between strains. RESULTS A total of 63 strains HMKP were collected, and the specimen were mainly from the emergency ICU and intra-neural ICU, both of which accounted for 33.33%（21/63）. The sputum specimens accounted for the highest proportion, 50.79%（32/63）, followed by blood specimens, 20.63%（13/63）. The HMKP strain is sensitive to most antimicrobial drugs. The serotypes were mainly K1 and K2, accounting for 39.68% and 30.16%, respectively. The K1 type virulence gene carrying mode was mainly rmpA+Kfu+Aer+iroN+entB+ybtS+fimH+mrkD+allS, accounting for 92.00%, K2 type was mainly rmpA+Aer+iroN+entB+fimH+mrkD, accounting for 47.37%. The main ST types included 21 strains of ST23 type, 7 strains of ST86 type, 4 strains of ST65 type, 4 strains of ST218 type. CONCLUSION HMKP often caused lower respiratory tract infections in intensive care patients in hospitals. Strict preventive measures and long-term monitoring should be established to control the prevalence of HMKP strains in these wards.