Abstract: OBJECTIVE To understand the distribution and drug resistance of detected A.baumanii from inpatients of a general hospital, so as to provide scientific basis for reasonable use of antibiotics. METHODS Totally 2 543 clinical isolates from clinical patients between 2011 and 2013 were collected. The test results and drug resistance of A. baumannii were retrospectively analyzed. Strains were identified by VITKE-2 Campact system and the drug sensitivity test was performed by K-B method. The software SPSS 17.0 was used for statistical analysis. RESULTS There were 2 543 A.baumanii strains isolated from the 25 758 pathogenic strains from the delivered specimens during the three years, with the detection rate of 9.9%. Specimens were mainly from departments of ICU, respiratory medicine and neurology medicine. The most A.baumanii were detected from sputum specimens. The total resistance rates in three years for A.baumanii were more than 80.0% to piperacillin/lazobactain, ampicillin/sulbactam, cftazidine, cefepime, cefuroxime, ceftriaxone, imipenema, tetracycline and ciprofloxacin, 79.5% to gentamicin and 78.5% to amikacin. The resistance rate was <20.0% to few antibiotics such as ampicillin, amoxicillin/clavulanic acid, cefazolin, aztreonam and nitrofurantoin. CONCLUSION Clinical distribution of isolated A.baumanii in the hospital mainly concentrated in ICU, the drug resistance was high and most strains were multi-drug resistant, so clinicians should strengthen the monitoring and prevention of A.baumanii infection and make further research on the resistance mechanisms.