Abstract: OBJECTIVE To explore the use of disk diffusion method in clinical laboratory screening of the OXA-type carbapenem-resistant Acinetobacter baumannii. METHODS A total of 32 strains of carbapenem-resistant A.baumannii were collected from the Affiliated Jinxiang Hospital of Jining Medical College from Jan 2012 to Jun 2014; the OXA-subtypes strains were screened out by using PCR and assigned as the test group; the sterile water, 1 strain of A.baumannii ATCC17978, 3 strains of IMP-type, and 3 strains of VIM-type carbapenem-resistant A.baumannii were set as four control groups. The oxacillin disc was put on the MH plate after the Staphylococcus aureus was coated, then the bacteria concentrations of all the groups were diluted to 0.5 Maxwell units ,and 10 μL of the diluted bacteria solution was added to the oxacillin disc, then the M-H plates were incubated at 35℃ for 48 hours, and the growth of bacterial colony in the oxacillin bacteriostatic circle was observed. RESULTS Totally 25 strains of OXA-type carbapenem-resistant A.baumannii were screened out by using the PCR and were assigned as the test group, the clear inhibition zones were observed around the oxacillin disk in the 5 groups after the culture for 12 hours, and there was no growth of bacterial colony in the bacteriostatic circle. There was growth of a few of bacterial colonies in the bacteriostatic circle of the test group after the culture for 24 hours. The bacterial colonies in the bacteriostatic circle grew bigger and were increased in the test group after the culture for 36 hours. There was no clear growth of bacterial colony in the bacteriostatic circle of the test group after the culture for 48 hours. There was no growth of bacterial colony in any of the four control groups during the whole process of culture. CONCLUSION The disk diffusion method can screen out the OXA-type carbapenem-resistant A.baumannii strains, and the optimal observation time is at the 36 hours of incubation under 35℃.