Abstract: OBJECTIVE To study the mechanism of action of hemolytic phospholipase C on pulmonary function of patients with Pseudomonas aeruginosa pulmonary infection so as to provide guidance for targeted treatment of the P.aeruginosa pulmonary infection. METHODS A total of 9 C57BI/6J male mice aged from 8 to 12 weeks were enrolled in the study and randomly divided into three groups: the PAO1group,the ΔplcH group , and the control gorup ( the PBS group), with 3 mice in each group. The 9 mice were used to establish the pulmonary infection models; the P.aeruginosa strains PA01 ( wild type, known as type WT) and the strains with deletion of allele gene ΔplcH were respectively cultured in LB medium so as to establish the mice pulmonary infection models. The ventilation and lung function of the mice were measured, the functions of the pulmonary surfactants were observed,and the statistical analysis of data was performed with the use of SPSS 19.0 software. RESULTS There was significant difference in the colony counts of P.aeruginosa in spleens or lungs between the PA01 group and the ΔplcH group after the mice were infected for 24 hours. The white blood cell counts in BALF were significantly less in the PBS group than in other two groups (P<0.05). The P-V curve and falling delayed inspiratory limb assessment results showed that the PAO1 group had very poor pathophysiology of lungs, as compared with the PBS group,the pulmonary function of the ΔplcH group was remarkably abnormal,but it was better than that of the PAO1 group. CONCLUSION The P.aeruginosa plcH may affect the lung function through influencing the pulmonary surfactants.