Abstract: OBJECTIVE To investigate the distribution characteristics,laboratory diagnosis,genotyping and drug resistance status of pathogenic fungi,so as to provide a guide for clinical diagnosis and rational drug use.METHODS Classification,drug susceptibility test and G test were analyzed in pathogenic fungi strains from specimens from Jan.2013 to Jan.2014,retrospectively.Polymerase chain reaction（PCR）technology was used to analyze transposable intron gene in 25 SrDNA,and automatic detection machine was used to perform identification and drug susceptibility tests for isolated pathogenic fungi.RESULTS Isolated pathogenic fungi were mainly found in patients’ sputum specimens,blood specimens,body fluids and pus,including 562 strains of Candida albicans（74.14%）,88 strains of Candida parapsilosis（11.61%）,49 strains of Candida glabrata.Fungi cultivation and G test were used in 164 patients with systemic fungal infection or fungal infection,the results of trial showed that the positive rate was 60.37% and 76.83%respectively.A type of Candida albicans was the most common in 190 patients with fungal infection,followed by B type,however,two strains were non-typeable.a higher resistance rate were also observed in A type of fungi compared with B type and C type of fungi to fluorine cytosine,itraconazole and voriconazole,as well as A,B,C types of fungi were resistant to fluconazole,except for amphotericin B.CONCLUSION Candidaare found in patients with respiratory tract,gastrointestinal tract and urinary tract infection.The resistance rate is different for Candidain diverse regions and genotypes,so that the results of rapid and accurate identification,drug susceptibility test,as well as genotyping have remarkable significance to diagnose patients with fungal infection in clinical.