Abstract: OBJECTIVE To understand the drug resistance genes in clinical Enterococcus faecium isolates and observe the characteristics of MLST genotyping so as to provide theoretical basis for reasonable use of antibiotics and control of nosocomial infections.METHODS A total of 112 strains of Enterococcus were isolated from Jan 2013 to Dec 2014, the strains were identified by using Phoenix microorganism automatic identification system, the drug resistance genes, including Van A, Van B, Van C1, Van C2/3, Van D, Van E, Van G, VanM, TetM, ermB, TEM、aac(6')-aph(2"), aph(3')-Ⅲ, and ant(6')-Ⅰ, were detected by means of PCR; 7 housekeeping genes, namely adk, atpA, ddl, gdh, purK, gyd, and pstS, were amplified so as to determine the sequences of the E.faecium. RESULTS Of 3 strains of vancomycin-resistant E.faecium, 2 were detected positive for Van A, 1 strain positive for Van B, 3 strains positive for ermB, 3 positive for aac (6')-aph(2"), 3 positive for aph (3')-Ⅲ. Totally 3 types of ST were identified through the MLST genotyping, the NO.1 strain was ST78, NO.2 strain and NO.3 strain were the novel ST types, which were ST1014 and ST1015, respectively.The drug susceptibility testing showed that the MIC of vancomycin was no less than 256 μg/ml, the MIC of teicoplanin no less than 256 μg/ml or 16 μg/ml, the MIC of linezolid 2 μg/ml. CONCLUSION The vancomycin-resistant E.faecium strains are increased; it is necessary for health care workers to take measures such as hand hygine, contact isolation, environmental disinfection, and reasonable use of antibiotics to control of VRE infection.