Abstract: HT5"SSMethicillin-resistant Staphylococcus aureus (MRSA) has become one of the major species of pathogens causing nosocomial infections since the MRSA infection was discovered, which brings more difficulties to the clinical treatment. This review defines the technologies of genotyping of MRSA, investigates the common methods including PFEG, Agr, multilocus sequence typing, spa, RM, SCCmec, and DNA Chip as well as the progress of latest genotyping technology, and make brief summaries of their respective characteristics so as to provide guidance for control of the MRSA infection. It is pointed out that no one genotyping assay can be used as the absolute index for the determination of correlation among the strains, on the contrary, it is better to choose two or more effective techniques to identify the strains according to their needs. With the continuous development of science and technology, the genotyping technologies of MRSA will promote the research of the MRSA.