Abstract: OBJECTIVE To explore the effect of nursing intervention of operating rooms on prevention of postoperative incision infections in patients undergoing total knee replacement so as to reduce the incidence of the postoperative infections in the patients undergoing the total knee replacement. METHODS A total of 2 000 patients who received the total knee replacement from Jun 2012 to Jun 2015 were enrolled in the study and randomly divided into the control group and the observation group, with 1 000 cases in each.The control group was treated with conventional nursing, while the observation group was given the modified nursing intervention, including monitoring and regulating the temperature in the operating room, keeping warm, and managing the surgical instruments.The rates of grade A incision healing and infection rates were observed and compared between the two groups of patients. RESULTS The rate of grade A incision healing of the observation group was 100.00%, significantly higher than 97.00% of the control group; the infection rate of the observation group was 0, significantly lower than 0.50% (5 cases) of the control group (P＜0.05). CONCLUSION It is necessary to take the operating room nursing interventions such as monitoring and regulating the intraoperative temperature in the operating rooms, keeping warm, and conducing the strict management of surgical instruments so as to remarkably raise the rate of grade A incision healing after the total knee replacement and reduce the infection rate.