Abstract: OBJECTIVE To observe the effect of frequency of bladder irrigation on urinary tract infections in puerpera with long-term indwelling catheter so as to reduce the incidence of urinary tract infections. METHODS A total of 300 puerpera who were treated with long-term indwelling catheter in department of obstetrics from Jan 2010 to Jan 2014 were enrolled in the study and randomly divided into the group Ⅰ, Ⅱ, Ⅲ, and Ⅳ, with 75 cases in each. The group Ⅰ group was treated with the bladder irrigation, twice a day; the group Ⅱ was treated with the bladder irrigation, once a day; the group Ⅲ was treated with bladder irrigation, twice a week; the group Ⅳ was not treated with bladder irrigation. All of the groups were treated with the same bladder irrigation. The midstream urine specimens were respectively cultured after the catheter indwelling for 3, 7, 14, and 21 days; the incidence of urinary tract infections was observed and compared among the 4 groups, and the statistical analysis was performed with the use of SPSS 18.0 software. RESULTS The incidence rate of urinary tract infections was 2.67% in the group Ⅰ on Day 3, 0 in the group Ⅱ, 0 in the group Ⅲ, 0 in the groupⅣ; the incidence rate of urinary tract infections was 10.66% in the group Ⅰ on Day 7, 9.33% in the group Ⅱ, 2.67% in the group Ⅲ, 10.66% in the group Ⅳ; the incidence rate of urinary tract infections was 16.00% in the group Ⅰ on Day 14, 21.33% in the group Ⅱ, 9.33% in the group Ⅲ, 24.00% in the group Ⅳ; the incidence rate of urinary tract infections was 36.00% in the group Ⅰ on Day 21, 34.67% in the group Ⅱ, 17.33% in the group Ⅲ, 42.67% in the group Ⅳ. The incidence rate of urinary tract infections of the group Ⅲ was signific antly lower than that of other three groups on Day 7, 14, or 21 of treatment, however, the incidence rate of urinary tract infections of the group signific antly reduce the incidence rate of urinary tract infections in the puerpera with long-term indwelling catheter and prevent the bacterial colonization.Ⅳ was significantly higher than that of other three groups CONCLUSION The bladder irrigation with the frequency of twice a week can significantly reduce the incidence rate of urinary tract infections in the puerpera with long-term indwelling catheter and prevent the bacterial colonization.