Abstract: OBJECTIVE To explore the prevalence of human herpes virus (EBV) and human papilloma virus (HPV) infections in patients with nose, paranasal sinuses inverted papilloma so as to understand the role of EBV and HPV in the nose, paranasal sinuses inverted papilloma. METHODS A total of 40 patients with nose, paranasal sinuses inverted papilloma who were treated in the hospital from Jun 2013 to Jun 2015 were recruited as the study objects. The EBV and HPV in the nose, paranasal sinuses papilloma paraffin-embedded tissues were detected by using polymerase-chain-reaction assay. Totally 40 patients with nose, paranasal sinuses inverted papilloma were assigned as the experimental group, including 10 cases of papilloma malignancy and 30 cases of nose, paranasal sinuses inverted papilloma. Totally 20 patients with nasal polyps were chosen as the control group. RESULTS The HPV was tested positive in 27 cases of the experimental group, with the positive rate 67.5%; the EBV was tested positive in 17 cases of the experimental group, with the positive rate 42.5%; the positive rates of HPV and EBV were significantly higher in the experimental group than in the control group (P<0.05). Of the 30 patients with nose, paranasal sinuses inverted papilloma in the experimental group, 14 had single HPV infection, 4 had single EBV infection, and 8 had mixed HPV and EBV infection. Of the 10 patients with papilloma malignancy, 5 had single HPV infection, 4 had single EBV infection, and 1 had mixed HPV and EBV infection. The incidence rate of HPV infection was 64.7% in the patients with recurrence, 65.2% in the patients without recurrence; the incidence rate of EBV infection was 47.1% in the patients with recurrence, 8.7% in the patients without recurrence; while there was significant difference in the incidence rate of EBV infection between the patients with recurrence and the patients without recurrence (P<0.05). CONCLUSION The EBV and HPV infections are prevalent in the patients with nose, paranasal sinuses inverted papilloma, and the two species of viruses are closely associated with the nose, paranasal sinuses inverted papilloma, to which the hospital should pay high attention.