Abstract: OBJECTIVE To explore the distribution and drug resistance of pathogens causing nosocomial infections in patients with cerebral infarction and put forward the prevention countermeasures for the nosocomial infections. METHODS A total of 120 cerebral infarction patients complicated with nosocomial infections who were treated in the hospital from Feb 2014 to Feb 2015 were enrolled in the study, then the sputum, blood, and urine specimens were collected for the bacterial culture, the constituent ratios of the pathogens and the infection sites were observed, and the statistical analysis of data was performed with the use of SPSS 16.0 software. RESULTS Of the 120 patients with nosocomial infections, 48 (40.0%) had lower respiratory tract infections. Totally 200 strains of pathogens were isolated, of which 68.0% (136 strains) were gram-negative bacteria. The drug susceptibility rates of the Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, and Staphylococcus aureus to imipenem, meropenem, and vancomycin were 100.0%. CONCLUSION It is an effective way to reasonably use antibiotics for the cerebral infarction patients complicated with nosocomial infections and take the effective prevention countermeasures so as to reduce the incidence of nosocomial infections.