Abstract: OBJECTIVE To investigate the possible contribution of multidrug resistant efflux pump gene to Candida albicans and Candida krusei azole resistance drugs, so as to provide theory basis for the drug resistance research of Candida spp. METHODS The drug resistant strains and sensitive strains of C. albicans and C. krusei to itraconazole were screened by drug sensitivity test in vitro, using real-time fluorescent quantitative PCR (FQ-RT-PCR) method to detect the mRNA expression level of multi-resistant efflux pump gene (CDR1/CDR2 /ABC1) in resistant and sensitive strains, and using rhodamine 6G as a tracer to test the efflux status in C. albicans and C. krusei. RESULTS The sensitive rates of C. albicans and C. krusei isolated from clinic to flucytosine and amphotericin B were relatively higher, all >80.00%, and the drug resistant rate of C. albicans to itraconazole was relatively higher >70.00%. After the statistical analysis to ΔCt numbers gotten from real-time fluorescent quantitative RT-PCR, it showed the expression quantity of CDR1, CDR2 mRNA from the drug resistant strains of C. albicans was significantly higher than that in sensitive strains (P＜0.05). However only one drug-resistant strain of C. krusei appeared, expression quantity of ABC1 mRNA had significant difference between sensitive strains and resistant strains . After the rhodamine 6G efflux test, the drug resistant strains would have significant increase in the expression of CDR1/CDR2 mRNA, as the efflux of rhodamine 6G increased as time goes. CONCLUSION Efflux pump gene CDR1 and CDR2 in C. albicans may play a role in the mechanism of drug resistance, but it cannot prove that there is no contribution of efflux pump gene ABC1 to the drug resistance in C. krusei, as the sample amount is not enough, which needs to expand the sample amount and give further study.