Abstract: OBJECTIVE To evaluate the diagnostic performance of PNA-FISH to rapidly and accurately identify Candida albicans of positive blood cultures. METHODS Blood culture positive specimens were simulated, and gram staining was used, and the type of pathogen was preliminarily identified. Two methods were used to identify the specimens which were displayed as Candida by microscopy, including PNA-FISH and traditional Chromagar Candida chromogenic medium identification technology. The specificity, sensitivity and detection time of the two methods were compared. RESULTS The specificity and sensitivity of PNA-FISH method for identification of C.albicans were 100% and 97.5%, and the detection time was (4±0.2) h, which was significantly shorter than (18~24)h of traditional method. CONCLUSION PNA-FISH is specific and sensitive for the identification of C. albicans of positive blood cultures, it needs shorter time, and is worthy of clinical promotion.