Abstract: OBJECTIVE To investigate all kinds of exotoxin genes carrying status in Staphylococcus aureus isolates in burn patients, so as to provide the basis for the treatment for S. aureus infection. METHODS S. aureus were isolated from the samples of our hospital burn patients from Jan. to Jun. 2015, using Vitek-2compact automatic identification device to give strain identification, using PCR to analysis exotoxin genes set1-26 and set30-40, and using Q type to have cluster analysis. RESULTS After using 37 kinds of exotoxin genes to detect 20 strains of S. aureus, only 1 strain was not detected any gene, and the remaining 19 strains had positive findings. Exotoxin genes only set2, set 4, set16, set23, set37, set39, etc had non positive findings, the positive rates for the rest were within 5.0% ~ 95.0%. There were 24 genes with positive rates more than 50%, 15 genes more than 75%. The detection results of exotoxin genes had diversity with 17 patterns. Cluster analysis showed that the 20 strains of S. aureus could be classified into big and small families. CONCLUSION There are 20 strains of S. aureus exotoxin genes with high positive rates, which may be related to the impetigo in burn wound and cellulitis.