Abstract: OBJECTIVE To investigate the prevalence rates of nosocomial infections and use of antibiotics so as to provide guidance for surveillance of the nosocomial infections. METHODS The cross-sectional survey was conducted for the patients were hospitalized from 0∶00 to 24∶00 on Sep 16, 2012, Sep 16,2013, and Sep 15, 2014; the unified questionnaires were filled out, and the statistical analysis was performed with the use of SPSS 13.0 software. RESULTS Totally 327 hospitalized patients were investigated in 2012, 340 cases in 2013, 348 cases in 2014; the prevalence rate of the nosocomial infections was 2.14% in 2012, 2.06% in 2013, 1.44% in 2014, and there was no significant difference in the prevalence rate of the nosocomial infections among the three years. The prevalence rate of the nosocomial infections was the highest (6.04%) in the internal medicine department 1; the patients with lower respiratory tract infections accounted for 38.10%, the patients with urinary tract infections 19.05%, the patients with incision infections 14.29%. The utilization rate of antibiotics was 33.10%, the patients treated with one-drug therapy accounted for 87.50%, the patients with two-drug therapy 11.90%, the patients with three-drug therapy 0.60%; the submission rate of specimens was 29.87%. CONCLUSION The internal medicine department is the key department to be monitored for the nosocomial infections, and the lower respiratory tract is the major infection site. It is necessary to strengthen the ward management, further standardize the aseptic operation and occupational protection of health care workers, reduce the invasive operations, and intensify the management of antibiotics so as to reduce the incidence of nosocomial infections.