Abstract: OBJECTIVE To explore the effect of bladder irrigation before extubation of urinary catheter on prevention and control of urinary tract infection in patients undergoing open surgery so as to provide guidance for prevention and control of the urinary tract infection in the patients undergoing the open surgery. METHODS A total of 120 urinary catheter indwelling patients who underwent the open surgery from Oct 2013 to May 2015 were enrolled in the study and randomly divided into the study group and the control group, with 60 cases in each group. The control group was not treated with bladder irrigation before the extubation, while the observation group was given the bladder irrigation before the extubation. The incidence rates of urinary tract infections and adverse reactions in the two groups of patients were observed and recorded after the nursing for 3, 7, and 14 days, the bacterial culture was carried out on day 3,7,and 14 of nursing, and the bacterial colony counts were recorded. RESULTS The incidence rate of urinary tract infection of the observation group was 1.67% after the treatment for 7 days, significantly higher than 10.00% of the control group; the incidence rate of urinary tract infection of the observation group was 3.33% after the treatment for 14 days, significantly lower than 23.33% of the control group (P<0.05). The incidence of adverse reactions in urethral orifice was 5.00% in the study group, 45.00% in the control group. The bacterial colony counts of Escherichia coli, Klebsiella spp, Proteus spp, Enterococcus faecalis, and other species of pathogens were significantly less in the study group than in the control group. The positive rate of bacterial culture of the study group was significantly lower than that of the control group on day 8, 12, or 16 after the urinary catheter indwelling (P<0.05). CONCLUSION The bladder irrigation can effectively reduce the incidence of urinary tract infection and the incidence of urethral orifice adverse reactions in the long-term urinary catheter indwelling patients.