Abstract: OBJECTIVE To survey antibiotic resistance of clinical isolates of carbapenem resistanct Acinetobacter baumannii (CRAB) in Yichang, and investigate molecular epidemiological characteristics of CRAB,so as to provide experimental evidence for the prevention and molecular epidemiology of CRAB infection. METHODS A total of 30 strains of CRAB from 4 general hospitals in Yichang from Oct.2013 to Oct.2014 were analyzed. Double-disk synergy test(DDST) was used to detect metallo-β-lactamase (MBLs),PCR was used to amplify carbapenemase genes(including OXA-23,OXA-24,OXA-51,OXA-58,IMP-1,IMP-2,VIM-1 and VIM-2), and the positive products were sequenced. RESULTS Of the 17 antibiotics tested, all strains were sensitive to polymyxin, the resistant rates to cefoperazone/sulbactam, minocycline were 26.7 % and 63.3% , and to other antibiotics were more than 80.0% . One strain of MBL phenotype was positive. Totally 28 strains amplified blaOXA-23 gene and one amplified blaVIM-2 gene. All the 30 strains were positive for the amplification of the OXA-51 gene, and no strain was found which carried OXA-24,OXA-58,IMP-1,IMP-2, or VIM-1 gene. CONCLUSION Multiple drug resistance is serious problems in Yichang. Production of OXA-23 and OXA-51 carbapenemases is an important mechanism of resistance to carbapenem antibiotics, and VIM-2 carbapenemases also cannot be ignored.