Abstract: OBJECTIVE To understand the prevalence of Brucellosis in Guangzhou area through the serological antibody test of fever patients with suspected Brucellosis in the past three years so as to provide scientific basis for prevention and control of Brucellosis. METHODS The serological antibody test was conducted for the fever patients who were conformed with suspected Brucellosis from 2012 to 2014, then 3-5 ml of venous blood samples were collected from the patients, the serum was separated and submitted to rose bengal plate agglutination test (RBPT) and standard agglutination test (SAT).The positive rates of detection of brucella antibodies were taken for statistics from 2012 to 2014, and the prevalence trend of Brucellosis in Guangzhou area was observed. RESULTS A total of 1 182 serum samples were collected from 2012 to 2014, of which 238 were tested positive, with the positive rate 20.1%.The positive rate of serological test of Brucellosis was 18.9% in Guangzhou in 2012, 19.6% in 2013, and 21.3% in 2014, showing an upward trend.The blood samples of the male patients with suspected Brucellosis were more than those of the female patients, and the positive rate of detection of brucella antibodies was higher in the male patients than in the female patients.The patients with suspected Brucellosis in the different age groups varied in the serological detection rate, the detection rate was the highest (27.3%) in the patients aged from 40 to 50 years old; the patients with different occupations varied in the detection rate, and the detection rate was 23.9% in the patients who engaged in the animal husbandry. CONCLUSION The positive rate of detection of Brucella antibodies is increased year by year in Guangzhou in the past three years, the prevalence of Brucellosis show an upward trend, the male patients are more than the female patients, the patients aged from 40 to 50 years old are dominant, and the patients who engage the animal husbandry are dominant.It is necessary to strengthen the prevention and control of epidemic of Brucellosis, monitor the high-risk populations, and conduct the publicity and education of Brucellosis among the practitioners.