Abstract: OBJECTIVE To evaluate the antibacterial drugs application and nutrition research on the influence of the intestinal fungus nosocomial infection patients with gynecological surgery in hospital, and to provide clinical prevention and treatment of intestinal fungus provide reference for the occurrence of iatrogenic infection. METHODS A total of 2 870 patients of gynecologic surgery in hospital from Jan. 2011 to Aug. 2011 were selected, and they were given antibiotics in the perioperative periods to prevent infections. The application and nutritional status of hospitalized patients with different antibiotics during the intestinal fungus iatrogenic infections were observed. Whether patients with intestinal fungal nosocomial infections was investigated, and stool inspection was timely carried out. The results were statistically analyzed by software SPSS19.0. RESULTS A total of 27 cases of postoperative intestinal fungus iatrogenic infection occurred with an infection rate of 0.94%. a total of 29 strains of fungi were detected, and they were mainly Candida albicans, Yeast, tropical Candida and Aspergillus with the constituent ratio of 48.27%, 27.59%, 17.24% and 6.90% respectively. When the conditions were controlled like these: time of antibacterial drugs application >3 d, types of antibacterial drugs >2, hemoglobin level ≤110 g/L and body mass index ≤18.5 kg/m², the infection rates of patients with intestinal fungus iatrogenic were all higher than that of these conditions: time of antibacterial drugs application ≤3 d, types of antibacterial drugs ≤2, hemoglobin level >110 g/L and body mass index >18.5 kg/m². The difference was significant (P<0.05). CONCLUSION C. albicans are the common pathogenic bacteria in department of gynaecology surgery in patients with intestinal fungus nosocomial infection, and intestinal fungus significantly increased risk of iatrogenic infection with longer time for using antibacterial drugs, more types of antibacterial drugs and poor nutritional status.