Abstract: OBJECTIVE To investigate antibiotic resistance,staphylococcal chromosomal cassette mec（SCCmec）genotypes,and Panton-Valentine leukocidin（PVL）gene in methicillin-resistant Staphylococcus aureus（MRSA）isolated from hospital infections,so as to make clear the MRSA resistant phenotypes and epidemiological characteristics and provide the reference for clinical treatment.METHODS MRSA was identified from 179 strains of S.aureus that were collected from 2011 to 2014.The drug resistance was detected by the K-B test.The genotypes of SCCmec were determined by multiplex PCR and the pvl gene was detected by simple PCR.RESULTS A total of106 strains of MRSA were identified from 179 strains of SAU,with the detection rate of 59.2%.The strains of MRSA were sensitive to vancomycin,quinupristin/dalfopristin,linezolid and teicoplanin completely.The drug resistant rates of MRSA to rifampin,compound sulfamethoxazole and nitrofurantoin were 43.4%,21.7% and4.8%,respectively,while to another antibiotics were above 50%.Among 106 isolates of MRSA,SCCmecⅢ,SCCmecⅡ,SCCmecⅣ and nontypeable isolates were accounted for 72.6%（77/106）,10.4%（11/106）,1.9%（2/106）and 15.1%（16/106）,respectively.Totally 4strains of MRSA were pvl gene positive,with the carrying rate of 3.8%.CONCLUSION S MRSA has got a serious multi-drug resistance,but no strain is resistant to vancomycin.The major SCCmec genotype of MRSA is SCCmecⅢ,and the secondary genotype is SCCmecⅡ.We should focus on the multi-drug resistant strains of SCCmec typeⅢ MRSA that carrying the pvl gene especially.