Abstract: OBJECTIVE To explore the value of echocardiography in diagnosis of patients with infectious endocarditis so as to provide guidance for clinical diagnosis and treatment. METHODS A total of 45 hospitalized patients with infectious endocarditis who underwent surgeries from Jan 2012 to Jan 2015 were recruited as the study objects, then the echocardiographic examinations and blood culture were conducted for the patients, and the results of the echocardiographic examinations were compared with the results of pathological examinations. RESULTS Totally 21 patients showed positive for blood culture, of whom 47.62% had Staphylococcus infection, and 38.10% had Streptococcus infection. The echocardiographic examination showed that 32 patients had neoplasms, of whom 75.76% had cardiac neoplasms with the diameter varying from 2 to 15mm, and 87.88% had no more than 3 cardiac neoplasms. The detection rates of valve prolapse, perivalvular leakage, and tendon rupture were 100.0%; the detection rates of abscess and valve perforation were 75.0% and 57.14%, respectively. CONCLUSION The echocardiography may facilitate the understanding of the locations, diameters, and quantities of the cardiac neoplasms of the patients with infectious endocarditis, which provides reliable basis for clinical diagnosis.