Abstract: OBJECTIVE To investigate the pathogenic bacteria in patients with incision infection after inguinal hernia repair and analyze the drug resistance, so as to decide antibacterial agents and management strategy. METHODS A total of 500 cases of inguinal hernia repair patients in our hospital from Sep. 2014 to Aug. 2015 received the study. The purulent secretions got from infected incision site were under bacterial culture and analysis of drug resistance. RESULTS A total of 25 cases of incision infection happened to the patients with inguinal hernia repair, and the infection rate was 5.00%, totally received 34 strains of pathogenic bacteria, mainly gram-positive bacteria 22 strains, accounting for 64.71%. The drug resistance to sulfamethoxazole and penicillin were both high, greater than 70.00%; all of the strains were susceptible to vancomycin. CONCLUSION The infection after inguinal hernia repair is mainly caused by gram-positive bacteria and the antimicrobial drugs should be adjusted based on the drug sensitive test of many antimicrobial agents, to better treatment for the incision infection of pathogenic bacteria after inguinal hernia repair patients.