Abstract: OBJECTIVE To investigate the utilization and drug resistance of antibacterial drugs from Jan.2012 to Dec.2014, so as to provide references for clinical rational use of antimicrobials. METHODS The data of antibacterial drugs usage from Jan.2012 to Dec.2014 were collected, and the detection rate was analyzed. Then the utilization of antibacterials was analyzed by using DDDs value and the data of drug resistance of clinical isolated pathogens were obtained from the laboratory department in our hospital, and the correlation between DDDs of antibacterials and drug resistance was analyzed by Pearson correlation analysis. RESULTS Totally 13362 strains of pathogens were isolated from Jan.2012 to Dec.2014 in the hospital. The isolated pathogenic bacteria mainly were gram-negative bacteria, and the proportions accounted for 58.0%, 59.3% and 58%, respectively in three years. Beta-lactam antibiotics were used predominantly in our hospital, and the drug resistance was fluctuated according to DDDs. The drug resistant rates of Acinetobacter baumannii (ICU)to imipenem was 89.4%, 85.1% and 80.6%, respectively, and Staphylococcus aureus to vancomycin and linezolid showed the sensitivity of 100% , but there was VRE existed in our hospital. The correlation coefficient r between the DDDs of levofloxacin and the resistant rate of E. coli (ESBL) was high (r＝0.997, P＜0.05). CONCLUSION Our hospital should continue to strengthen the management of clinical application of antibacterial drugs and the monitoring of drug resistance, so sa to promote the clinical rational use of antimicrobial drugs.