Abstract: OBJECTIVE To study pathogen distribution and drug resistance of infectious keratitis, so as to provide a basis for clinical treatment. METHODS Clinical data of 87 cases of infectious keratitis patients in the hospital from Dec.2012 to Dec. 2015 were selected and analyzed. The specimens of patients were collected and cultured, drug sensitivity test was using a modified KB disk diffusion method according to NCCLS test results interpretation criteria, and the pathogen distribution and drug resistance of infectious keratitis were analyzed. RESULTS Totally 28 strains of pathogens were isolated from 87 cases of infectious keratitis patients, including 15 strains of gram-positive bacteria, accounting for 53.58%, and 1 strain of gram-negative bacteria, accounting for 3.57%. 3 cases of gram-positive bacteria, 10.71% 9 gram-negative bacteria, accounting for 32.14%. The drug resistant rates of gram-positive bacteria Staphylococcus epidermidis to penicillin G, ampicillin and imipenem were 83.33%, 66.67% and 50.00%, Staphylococcus aureus to penicillin G and imipenem were 100.00% and 66.67%, Streptococcus pneumoniae to penicillin G was 100.00%, E. coli to ampicillin and cefuroxime were both 100.00%, Pseudomonas aeruginosa to all drugs were over 50.00%, and to ampicillin reached 100.00%, Klebsiella pneumoniae to ciprofloxacin, cefazolin and ceftazidime were was all 0. CONCLUSION Patients with infectious keratitis had many types of pathogens, mainly E. coli and S. aureus, and it should strengthen the monitoring of pathogens, and choose the right antibiotic therapy, to improve the cure rate.