Abstract: OBJECTIVE To explore the clinical application value of urine biomarkers in children with kidney injury induced by cytomegalovirus ( CMV) infection and analyze the study significance so as to provide guidance for clinical research. METHODS A total of 56 children with kidney disease who were treated in the hospital from Jan 2014 to Jan 2015 were assigned as the kidney disease group, meanwhile, 50 healthy children were set as the control group. The IgG, α1-MG, β2-MG, m-ALB and NAG value of all the subjects were determined; the combined biological levels were observed and compared among the groups. RESULTS There was significant difference in the level of α1-MG, β2-MG, or NAG between the kidney disease group and the control group (P<0.05); there was significant difference in the level of α1-MG, β2-MG, or NAG in the kidney disease group before and after the treatment (P<0.05). As for the combined use of kidney injury markers, the positive rate was 100.00% in the kidney disease group, 0 in the control group. CONCLUSION The combined detection of IgG, α1-MG , β2-MG, m-ALB, and NAG may facilitate the prevention and indication of the kidney injury induced by CMV infection in the children.