Abstract: OBJECTIVE To analyze clinical diagnosis value of the combined detection to peripheral blood alanine aminotransferase (ALT), aspartate aminotransferase (AST) and gammaglutamyltransferase (GGT) on various types of liver disease, so as to provide reference for identification and diagnosis to liver disease. METHODS A total of 184 patients with liver disease admitted in our hospital from Jun. 2012 to Jun. 2015 were selected as research group, and 50 cases of healthy physical examinees in the same period were enrolled in the control group. The levels of peripheral blood ALT, AST and GGT were compared between the two groups, and the difference of positive rates of ALT, AST and GGT were observed in all kinds of liver disease patients. RESULTS The levels of ALT AST and GGT in patients with acute viral hepatitis and severe viral hepatitis were significantly higher than those in control group. The levels of GGT and AST in peripheral blood in patients with hepatitis cirrhosis and primary hepatic carcinoma were significantly higher than those in control group (P<0.05). The positive rates of GGT, AST and ALT in the peripheral blood of each group were consistent with the difference of the expression level. APTT, TT and PT in patients with primary liver cancer were significantly higher than those in the control group; The APTT and TT in patients with hepatitis cirrhosis were significantly higher than those in the control group; FIB in patients with liver disease was significantly lower than that in the control group (P<0.05). CONCLUSION The combined detection of FIB, GGT, AST and ALT levels in peripheral blood could help to provide reliable laboratory data for clinical identification and diagnosis of liver diseases with good diagnosis value.