Abstract: OBJECTIVE To explore the application effect of reinforcement of sterilization and isolation management in the prevention of infection in interventional operation rooms, so as to effectively prevent the hospital infections in patients with interventional operation. METHODS Patients were selected for the study of interventional diagnosis or treatment for the study, the hospital began to implement the infection sources to strengthen the disinfection and isolation management since Jan. 2013, before that(Jan. 2011 to Dec. 2012) a total of 1530 cases and after that (Jan, 2013 to Dec. 2014) a total of 1500 cases of patients were chosen and compared about the infection rates, and bacterial changes and distribution of pathogens 10min before operation, in 10min, 60min and the end. RESULTS Before the isolation management of disinfection, the infection rate of interventional operation patients was 10.39%, which was significantly higher than after 0.93% (P<0.01). After the implementation of the infection source to strengthen the disinfection and isolation management, the bacterial count was significantly lower than before (P<0.01). The top 3 of the infection rate of the pathogens were Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus after the management, which were significant different than before (P<0.01). CONCLUSION Strengthening disinfection management of infection sources can reduce the number of bacteria in operation rooms at different times, reduce the incidence of nosocomial infections in patients with interventional therapy.