Abstract: OBJECTIVE To investigate the influence of infection control and supervision and management from intervention staff to surgical infections in patients, in order to prevent hospital infections effectively. METHODS Patients underwent surgery in the same operation room from Jul. 2011 to Jun. 2013 and from Jul. 2013 to Jun. 2015 were selected and divided into control group and intervention group, 1546 cases in the observation group and 1512 cases in the control group, two groups of patients with same medical personnel. The control group was given conventional treatment, and the intervention group was supervised and managed by the infection control intervention staff. Hand hygiene compliance rate of two groups medical personnel, infection rate of patients and antimicrobial drug use time were compared between the two groups. The indoor change of bacteria and pathogenic bacteria distribution at 10 minutes before operation, 10 min and 60 min of operation and the end of operation were compared. RESULTS The hand hygiene execution rate of medical staff in control group was 60.38%, which was lower than that in intervention group 97.83%. The infection rate after operation in control group was 0.91%, and intervention group 8.0%. The antimicrobial drug use time in intervention group was (2.23±0.42)d, which was shorter than control group (4.88±1.21)d . All the comparison above showed significant difference (P<0.01). Bacteria amount in interior of operation room in all time periods of intervention group was significantly lower than control group (P<0.01); A total of 14 strains of pathogens were isolated from intervention group and control group 121 strains, and the top three were Escherichia coli, Klebsiella pneumoniae and Staphylococcus aureus. CONCLUSION The number of bacteria in the operation rooms can be significantly reduced by infection control intervention staff, which could also shorten the time of using antimicrobial agents and improve the implementation rate of hand hygiene, so as to reduce the incidence of hospital infections in patients with surgery.