Abstract: OBJECTIVE To investigate the application of hemofiltration in treatment of severe abdominal infection, and investigate its effects on plasma endotoxin and cytokines, so as to provide a reference guide for the disease. METHODS A total of 40 cases of severe abdominal infection patients in the hospital from Jan,2014 to Dec.2015 were selected for the study, patients were taken by continuous intravenous hemofiltration, and changes of plasma endotoxin and cytokines were observed before treatment and 48 h and 72 h after treatment. RESULTS There were 40 strains of pathogens isolated from 40 cases of severe abdominal infection patients, including 30 strains of gram-negative bacteria and 10 strains of gram-positive bacteria, accounting for 75.0% and 25.0%. The clinical total effective rate was 92.5%.The endotoxin, TNF-α, and IL-10 were (63.5±10.7) ng/L, (441.6±34.8) ng/L, and (126.4±9.5) ng/L before treatment, respectively, were (41.5±7.7) ng/L, (277.3±15.5) ng/L, and (58.4±6.4) ng/L 48 h after treatment, respectively, and were (36.8±4.8) ng/L, (247.3±13.8) ng/L, and (50.1±5.8) ng/L 72 h after treatment, respectively. The endotoxin, TNF-α, and IL-10 48 h and 72 h after treatment were significantly lower than those before treatment(P<0.05). CONCLUSION The treatment of severe abdominal infection with hemofiltration in clinical is feasible, it can remove plasma endotoxin and cytokines, and is worthy of clinical application.