Abstract: OBJECTIVE To investigate the effects of different types,timing and scheme of antibiotics on the postoperative wound infection in department of general surgery patients,in order to provide guidance for rational use of antibiotics in department of general surgery.METHODS A total of 300 cases of clinical data of patients in general surgery by operation treatment in our hospital from Jan.2014 to Dec.2015 were retrospectively analyzed,who were divided into three groups A,B,C according to antibacterial drugs application time,group A with 86 cases,using antibacterial drugs preoperative 1 2H,group B with 112 cases,using antibacterial drugs preoperative0 H,and group C with 102 cases,using antimicrobial agents during or after operation.The incidence of incision infection in three groups were observed after operation.RESULTS Cephalosporins were the main antimicrobial agents in these three groups,followed by penicillins,quinolones and nitroimidazoles,which were all using the way of drug combination,accounting for 84.88%in group A,81.25%in group B and 79.41%in group C,and there were no significant difference in three groups of antimicrobial drug use.The incision infection rate in group A was5.81%,group B 6.25%,group C 14.71%,and there was significant difference in these three groups（P＜0.05）.There were 17 cases of incision infection stop using antimicrobial drugs 1Dafter operation,10 cases 2Dafter operation,1case 3D or 5D after operation,and the difference was significant in three groups（χ2=25.548,P＜0.05）.The incidence of incision infection rates 1Dand 2Dafter operation with single application of antibacterial drugs had no significant difference,but higher than that after 3D,5Dof operation with significant difference（χ2=7.501,P＜0.05）.The incidence of incision infection rates 1Dand 2Dafter operation with drug combination therapy had no significant difference,but higher than that after 3D,5Dof operation with significant difference（χ2=5.042,P＜0.05）.CONCLUSION General surgical patients with antibacterial drugs should follow the basis of the Guidelines for Clinical Use of Antibacterials but with personalized use,and pay attention to the latest and local wound infection etiological analysis and drug sensitivity test to reduce the general surgery incision infection rate.