Abstract: OBJECTIVE To investigate the clinical distribution and antibiotic resistance of Haemophilus influenza,so as to provide references to physicians for making reasonable prescriptions.METHODS The distribution,identification and antimicrobial susceptibility testing of all strains isolated from Jan.2006 to dec.2015 were analyzed.The results of antimicrobial susceptibility testing and resistant phenotypes were analyzed by WHONET 5.6.RESULTS Totally 1671 strains of H.influenza were isolated from 2006 to 2015in our hospital,and 86.3%（1442/1671）of the strains came from sputums.The sensitive rates of H.influenzaisolated from patients of children and adults to sulfamethoxazole/trimethoprim were the lowest,accounting for 33.8% and 47.1%,followed by ampicillin of 46.7% and 65.4%,and the sensitive rates were all above 70.0% to other antibiotics.CONCLUSION Most of the strains of H.influenzae in our hospital were isolated from respiratory tract.TMP/SMZ and ampicillin were not suitable for experiential therapy of H.influenzaeinfection.Physicians should choose antibiotics according to the antimicrobial susceptibility test.