Abstract: OBJECTIVE To investigate influencing factors of vaginal pathogen infection in patients with cervical intraepithelial neoplasia（CIN）,so as to provide a basis for clinical practice.METHODS Clinical data of 620 cases of CIN patients in the hospital from Dec.2010 to Dec.2015 were analyzed.The patients were received routine examination and cervical LEEP surgery treatment after admission.Self questionnaire of influencing factors of vaginal pathogen infection was used,and univariate and multivariate unconditional logistic regression analysis was conducted.RESULTS There were 58 cases occurred vaginal pathogen infection in 620 cases CIN patients,and the infection rate was 9.35%.Totally 89 strains of pathogens were isolated,the top two were human papillomavirus（HPV）and Chlamydia trachomatis（Ct）,accounting for 40.45% and 23.60%.Univariate analysis and multivariate unconditional logistic regression analysis showed that age,cervical intraepithelial neoplasia grade,postoperative positive margin and persistent HPV infection after conization were closely related with vaginal pathogen infection of CIN patients,and the differences were significant（P＜0.05）.CONCLUSION Vaginal pathogen infection in patients with cervical intraepithelial neoplasia has many risk factors,postoperative patients should be strengthened pathogens and drug resistance tests,so as to reduce infection rates.