Abstract: OBJECTIVE To study the effects of enteral nutrition at different times on intestinal infection in acute pancreatitis (SAP) patients, so as to provide a theoretical basis for future clinical treatment. METHODS A total of 68 patients with acute pancreatitis treated in our hospital from Dec. 2012 to Dec. 2015 were selected, which were divided into three groups, including group A of 23 patients in the first two days of admission enteral nutrition, group B of 23 patients in 4 days after admission started enteral nutrition, and group C of 22 patients hospitalized in the sixth day of enteral nutrition. Physiological changes, incidence of adverse reactions and intestinal source of infection of three groups of patients in each period were compared. RESULTS Five days after admission, the WBC, C- reactive protein and endotoxin of group A were significantly decreased, and were decreased to (10.3±1.2)×10⁹/L, (55.0±14.0) mg/L and (0.30±0.08) EU/L in the ninth day, the degree of decline was significantly less than that of group B and group C (P<0.05). There were 1, 2, and 4 cases of intestinal infection in group A, B, and C, accounting for 4.4%, 8.7% and 18.2%, respectively, and had no significant difference in adverse events. CONCLUSION In patients with acute pancreatitis admitted the first two days of enteral nutrition can well reduce inflammation of the intestinal infection, plays a preventive role in development of intestinal infection, which is a reliable and efficient method of treatment.