Abstract: OBJECTIVE To investigate the pathogens distribution and resistance of nosocomial infection in patients of department of gastroenterology, so as to provide theoretical guidance and basis for the clinical treatment of hospital infection of gastrointestinal hospitalized patients. METHODS A total of 180 cases of gastrointestinal patients with hospital infection from Feb. 2014 to Jun. 2015 were selected as study objects, and a retrospective survey method on clinical data of 180 cases was carried out. The types of pathogens were analyzed, and susceptibility experiment was carried out to analyzed the resistant rate. RESULTS Totally 296 strains of pathogens were isolated from 180 cases of infected patients, including 112 strains of gram-positive bacteria and 184 strains of gram-negative bacteria, accounting for 37.84% and 62.16%, respectively. Staphylococcus haemolyticus, Staphylococcus aureus and Enterococcus had the high resistant rate to penicillin G, accounting for 92.86%, 91.67% and 94.44%, respectively. Pseudomonas aeruginosa, Acinetobacter baumannii, and Klebsiella pneumoniae had the high resistant rate to ciprofloxacin, accounting for 68.75%, 64.29% and 61.54%, respectively. CONCLUSION Gastrointestinal inpatients have long hospital stay, complex treatment, combined with their physical insufficiency and low immunity, so they are prone to infection, and the infection rate is very high, it is worth focusing on clinical treatment.