Abstract: OBJECTIVE To analyze the epidemic characteristics of multidrug resistant organisms,so as to provide medical evidence for prevention and control of multidrug-resistance organism（MDRO）infection.METHODS A total of 7443 samples from hospitalized patients were collected from Jan.2013 to Apr.2014.Automatic bacteria analyzer（VITEK2Compact,Merieux Company,France）was used to analyze the samples.And all data were analyzed by using SPSS17.0.RESULTS A total of 1769 strains of samples were positive from the 7443 strains of samples,and the positive rate was 23.77%.A total of 666 strains of MDRO were isolated and the detection rate was37.65%.The main MDRO was 320 strains of ESBLs producing Escherichia coli,accounting for 48.04%,followed by 152 strains of MDRO Acinetobacter baumanni,accounting for 22.08%.In different departments,there were obvious characters for MDRO,the A.baumannii accounted for 57.89% in ICU,and ESBLs producing E.coli accounted for 45.94%in surgery systems,MRSA with wound surface department of orthopaedics,burn department accounted for 48.84%.CONCLUSION The MDRO could be predicted by infection sites,the basic status of patients and departments.