血液感染多药耐药鲍氏不动杆菌的同源性分析
Homological analysis of multidrug resistant Acinetobacter bamannistrains in bloodstream infections
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摘要: 目的 通过对引起血液感染的多药耐药鲍氏不动杆菌(MDRAB)临床分离株进行脉冲场凝胶电泳(PFGE)分子分型分析,探讨各耐药菌株之间的同源性,了解MDRAB的流行和分布特征。方法 收集医院2007年4月-2013年6月引起血液感染MDRAB临床分离株95株,采用PFGE技术并结合Bionumerics Applied Maths软件分析各耐药菌株之间的同源性。结果 95株菌株分为A-N共14型克隆株,以克隆C、E、J为主,分别占20.00%、29.47%、20.00%,主要分布于呼吸内科病区、重症监护室和急诊病房,从克隆的时间分布显示,大部分克隆存在散在流行。结论 2007年4月-2013年6月期间引起血液感染的MDRAB有14型克隆株,其中以克隆C、E、J为主,主要分布于呼吸内科病区、重症监护室和急诊病房,应在这些科室展开医院感染目标性监测和控制。Abstract: OBJECTIVE To analyze the molecular types of multidrug resistant Acinetobacter bamannii(MDRAB)strains causing bloodstream infections by pulsed field gel electrophoresis(PFGE),so as to investigate the homology of these drug resistant strains,and comprehend the epidemic and distribution characteristics.METHODS Totally95 strains of MDRAB strains causing bloodstream infections were collected from the patients of the general hospital of Chinese People′s Liberation Army from Apr.2007 to Jun.2013,and the homology of these strains were analyzed by PFGE and Bionumerics Applied Maths software.RESULTS Ninety-five strains of MDRAB were divided into 14 clonal strains named A to N,and the primary clonal strains were C,E,and J,accounting for 20.00%,29.47%,and 20.00%,respectively,which mainly spread across the Respiratory,ICU and Emergency Department.The time distribution of these clonal types showed that most of them were epidemic sporadically.CONCLUSION There were 14 clonal strains of MDRAB causing bloodstream infections from Apr.2007 to Jun.2013,and the primary clonal strains were C,E,and J,mainly spreading across the Respiratory,ICU and Emergency Department.We should monitor goal-directedly and strengthen the control of nonsocomial infection.
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