Abstract: OBJECTIVE To investigate the testing results of hepatitis B virus (HBV) serologic markers in school-age children undergoing physical examination in Zunyi area and understand the effect of immunization in local area so as to provide guidance for deployment of future work. METHODS A total of 1 200 school-age children who received physical examination from Mar 2015 to Feb 2016 were enrolled in the study, then the fasting blood samples were collected for the testing of HBV serologic markers, the positive rates of detection of HBV surface antigen (HBsAg), HBV surface antibody (HBsAb), HBV e antigen (HBeAg), HBV e antibody (HBeAb), and HBV core antibody (HBcAb) were observed and compared among the children in the different genders, age groups, and places of residence, and the children whose parents carried with HBV. RESULTS The positive rate of detection of HBsAb of the male children was higher than that of the female children (P＜0.05).The positive rates of detection of HBsAg of the children in rural area was lower than that of the children in urban area (P＜0.05).The negative rates of detection of all the markers were lower in the children in rural area than the children in urban area (P＜0.05).The positive rate of detection of HBsAg, positive rate of detection of HBsAb, and negative rates of detection of all the markers were lower in the children aged no more than 9 years old than in the children aged between 9 and 12 years old (P＜0.05).The positive rate of big san yang, positive rate of small san yang, positive rate of HBsAg, positive rate of HBsAb, and negative rates of all markers were higher in the children whose parents carried with HBV than in the children whose parents did not carry with HBV (P＜0.05). CONCLUSION The hepatitis B vaccination had achieved satisfied effect in Zunyi area, and the next step is to focus on the enhancement of reexamination of the vaccination effect as well as blocking mother-to-infant transmission.