Abstract: OBJECTIVE To discussion the expression and correlation of LN（laminin,LN）and hepatitis b surface antigen（HBsAg）in the tissues of HBV-related hepatocellular carcinoma,so as to provide reference for clinical diagnosis and treatment of HBV-related hepatocellular carcinoma.METHODS A total of 50 cases of patients with HBV-related hepatocellular carcinoma and 50 cases of patients with benign liver diseases from Jan.2014 to Dec.2015 in the hospital were selected.Immunohistochemical method was used to detect the expression of LN and HBsAg in tissues of hepatocellular carcinoma and benign liver diseases,and normal tissue adjacent to carcinoma,and chemiluminescence was used to determine serum LN.RESULTSIn Hepatocellular carcinoma and normal tissue adjacent to carcinoma tissue,benign liver disease,LN expression positive rate were 28.00%,8.00% and 4.00%in membrane/cytoplasm,LN expression positive rate were 66.00%、54.00% and 8.00% in hepatic sinus and new blood vessels,LN expression positive rates in membrane/cytoplasm and hepatic sinus and new blood vessels with hepatocellular carcinoma were higher than those of normal tissue adjacent to carcinoma tissue and benign liver disease（P＜0.05）,LN expression positive rates in hepatic sinus and new blood vessels with hepatocellular carcinoma and normal tissue adjacent to carcinoma tissue and benign liver disease were higher than those of membrane/cytoplasm（P＜0.05）,and serum LN in hepatocellular carcinoma were higher than that of benign liver disease（P＜0.05）.HBsAg expression positive rates were 42.00%,70.00% and 12.00%in hepatocellular carcinoma,normal tissue adjacent to carcinoma tissue and benign liver disease,HBsAg expression positive rate of normal tissue adjacent to carcinoma tissue was higher than that of hepatocellular carcinoma and benign liver disease（P＜0.05）,and HBsAg expression positive rate of hepatocellular carcinoma tissue was higher than that of benign liver disease（P＜0.05）.LN and HBsAg positive expression rate was positive correlated with hepatocellular carcinoma tissue（P＜0.05）,LN and HBsAg positive expression rate was positive correlated with normal tissue adjacent to carcinoma tissue（P＜0.05）,and LN the positive expression rate and serum levels of hepatocellular carcinoma tissue and normal tissue adjacent to carcinoma tissue had positive correlation（P＜0.05）.CONCLUSION LN in the tissues and the serum level of expression are raised with hepatocellular carcinoma tissue and normal tissue adjacent to carcinoma tissue,HBV infection may be induced to normal liver cell development as the main factors of hepatocellular carcinoma,and LN may participate in the development and the transfer process of liver cancer.