Abstract: OBJECTIVE To statistically analyze the drug resistance of Acinetobacter baumannii strains isolated from clinical laboratory department in recent three years so as to provide guidance for surveillance of regional drug resistance and clinical use of antibiotics. METHODS From Jan 2013 to Dec 2015, the specimens were collected from the patients with infection. The bacterial cultivation and the drug susceptibility testing were conducted. The CLSI breakpoints were updated year by year, and the susceptibility result (susceptible intermediate resistant) of the A.baumannii strains to designated antibiotics were determined and evaluated.In addition, statistical analysis of data was performed with the use of WHONET5.6 software. RESULTS Totally 363 strains of A.baumannii were isolated in the three years, of which 99 (27.3%) were isolated in 2013, 126 (34.7%) in 2014, 138 (38.0%) in 2015. The isolation rate of the A.baumannii strains was highest in respiratory tract, followed by urinary tract. The drug resistance rate of the A.baumannii strains to the commonly used antibiotics generally showed an upward trend in the three years; the drug resistance rates to polymyxin B, minocycline, and cefoperazone-sulbactam were less than 20%, however, the drug resistance rates to sulfamethoxazole-trimethoprim, gentamicin, ciprofloxacin, piperacillin, and ceftazidime were more than 50%; the strains resistant to polymyxin B had not been detected. CONCLUSION The incidence rate of nosocomial infection caused by the epidemic A.baumannii strains is increased rapidly in the hospital of Jianghan Plain. It is necessary for the hospital to prevent rapid increase of the drug resistance of the epidemic strains and try to improve the level of treatment of infectious diseases.